{"id":7055,"date":"2025-10-15T12:42:05","date_gmt":"2025-10-15T10:42:05","guid":{"rendered":"https:\/\/www.techscale.cz\/cs\/?p=7055"},"modified":"2025-10-15T12:42:05","modified_gmt":"2025-10-15T10:42:05","slug":"revolucni-senzor-dokaze-menit-ph-uvnitr-zivych-bunek","status":"publish","type":"post","link":"https:\/\/www.techscale.cz\/en\/2025\/10\/15\/revolucni-senzor-dokaze-menit-ph-uvnitr-zivych-bunek\/","title":{"rendered":"Revolutionary sensor can measure pH inside living cells with unprecedented accuracy"},"content":{"rendered":"[vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” column_direction=”default” column_direction_tablet=”default” column_direction_phone=”default” scene_position=”center” text_color=”dark” text_align=”left” row_border_radius=”none” row_border_radius_applies=”bg” overflow=”visible” overlay_strength=”0.3″ gradient_direction=”left_to_right” shape_divider_position=”bottom” bg_image_animation=”none” gradient_type=”default” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_tablet=”inherit” column_padding_phone=”inherit” column_padding_position=”all” column_element_direction_desktop=”default” column_element_spacing=”default” desktop_text_alignment=”default” tablet_text_alignment=”default” phone_text_alignment=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_backdrop_filter=”none” column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/1″ tablet_width_inherit=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid”][vc_column_text]\n

A new generation of nanosensors capable of detecting pH inside living cells with previously unseen sensitivity and stability has been developed by a team of scientists from CATRIN, Palack\u00fd University Olomouc, and VSB \u2013 Technical University of Ostrava, in cooperation with colleagues from Brno University of Technology. The Czech researchers presented the carbon-dot-based sensors, which offer great potential for medical diagnostics, in the journal Biosensors and Bioelectronics. <\/a><\/strong><\/h2>\n[\/vc_column_text][divider line_type=”No Line” custom_height=”25″][vc_column_text]The pH value is one of the most frequently measured chemical parameters, but the development of nanometric sensors capable of accurately mapping the distribution and dynamics of pH with high spatial and temporal resolution remains a significant challenge. Such sensors are important for our understanding of numerous physiological and pathological processes. The pH value, among other things, has a fundamental effect on enzyme function and influences metabolic and signalling pathways as well as the transport of substances across membranes.<\/p>\n

\u201cWe were looking for a way to monitor processes inside cells with much higher precision than current probes allow. We have developed a sensor that is small, brightly luminescent, highly stable, and capable of tracking pH changes over an exceptionally wide range. The sensor not only glows under the microscope but also detects pH changes based on fluorescence lifetime, which is a more accurate and reliable parameter than ordinary light intensity,\u201d explained Sergii Kalytchuk, the first author of the study, from CATRIN and the Materials-Environmental Laboratory (MEL) at VSB\u2013TUO.[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” column_direction=”default” column_direction_tablet=”default” column_direction_phone=”default” scene_position=”center” left_padding_desktop=”0″ constrain_group_2=”yes” right_padding_desktop=”0″ text_color=”dark” text_align=”left” row_border_radius=”none” row_border_radius_applies=”bg” overflow=”visible” overlay_strength=”0.3″ gradient_direction=”left_to_right” shape_divider_position=”bottom” bg_image_animation=”none” gradient_type=”default” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_tablet=”inherit” column_padding_phone=”inherit” column_padding_position=”all” column_element_direction_desktop=”default” column_element_spacing=”default” desktop_text_alignment=”default” tablet_text_alignment=”default” phone_text_alignment=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_backdrop_filter=”none” column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/2″ tablet_width_inherit=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid” column_padding_type=”default” gradient_type=”default”][image_with_animation image_url=”7057″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”center” border_radius=”10px” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][\/vc_column][vc_column right_padding_desktop=”5%” column_element_direction_desktop=”default” column_element_spacing=”default” desktop_text_alignment=”default” tablet_text_alignment=”default” phone_text_alignment=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_backdrop_filter=”none” column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/2″ tablet_width_inherit=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid” column_padding_type=”advanced” gradient_type=”default”][vc_column_text]In contrast to other fluorescent pH sensors, which suffer from gradual photobleaching, chemical degradation, and often require calibration, the new nanosensors maintain excellent performance even under demanding conditions. Their fluorescence lifetime predictably increases with rising pH, which enables direct measurement of cellular acidity \u2013 a key parameter closely related to pathological processes such as cancer and neurodegeneration.<\/p>\n

\u201cIn an experiment with human skin cells, and using advanced microscopy based on fluorescence lifetime imaging (FLIM), the sensors enabled precise mapping of pH inside lysosomes, which are, simply put, the cell\u2019s recycling and cleaning units. Moreover, it was possible to detect pH changes after the application of chemical inhibitors, which opens possibilities for drug testing and for monitoring cellular stress in real time,\u201d said Tom\u00e1\u0161 Malina, another author of the study.<\/p>\n

The development of the sensor took place at the interface of synthetic chemistry and physics, but its application in biomedicine gives the entire work practical significance.<\/p>\n

\u201cThe study shows how basic materials chemistry can be linked with real biomedical challenges. Thanks to their exceptional photostability, biocompatibility, and extremely wide pH range, these sensors have great potential to become an effective tool for medical diagnostics, including clinical applications,\u201d concluded Radek Zbo\u0159il, the corresponding author of the paper.[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” column_direction=”default” column_direction_tablet=”default” column_direction_phone=”default” scene_position=”center” text_color=”dark” text_align=”left” row_border_radius=”none” row_border_radius_applies=”bg” overflow=”visible” overlay_strength=”0.3″ gradient_direction=”left_to_right” shape_divider_position=”bottom” bg_image_animation=”none”][vc_column column_padding=”no-extra-padding” column_padding_tablet=”inherit” column_padding_phone=”inherit” column_padding_position=”all” column_element_direction_desktop=”default” column_element_spacing=”default” desktop_text_alignment=”default” tablet_text_alignment=”default” phone_text_alignment=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_backdrop_filter=”none” column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/1″ tablet_width_inherit=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid”][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”full_width_content” full_screen_row_position=”middle” column_margin=”default” column_direction=”default” column_direction_tablet=”default” column_direction_phone=”default” scene_position=”center” top_padding=”2%” left_padding_desktop=”36%” constrain_group_2=”yes” right_padding_desktop=”36%” text_color=”dark” text_align=”left” row_border_radius=”none” row_border_radius_applies=”bg” overflow=”visible” overlay_strength=”0.3″ gradient_direction=”left_to_right” shape_divider_position=”bottom” bg_image_animation=”none” gradient_type=”default” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_tablet=”inherit” column_padding_phone=”inherit” column_padding_position=”all” column_element_direction_desktop=”default” column_element_spacing=”default” desktop_text_alignment=”default” tablet_text_alignment=”default” phone_text_alignment=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_backdrop_filter=”none” column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/1″ tablet_width_inherit=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid”][image_with_animation image_url=”6651″ image_size=”full” animation_type=”entrance” animation=”None” animation_movement_type=”transform_y” hover_animation=”none” alignment=”” border_radius=”none” box_shadow=”none” image_loading=”default” max_width=”100%” max_width_mobile=”default”][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” column_direction=”default” column_direction_tablet=”default” column_direction_phone=”default” scene_position=”center” top_padding=”2%” left_padding_desktop=”10%” constrain_group_2=”yes” right_padding_desktop=”10%” text_color=”dark” text_align=”left” row_border_radius=”none” row_border_radius_applies=”bg” overflow=”visible” overlay_strength=”0.3″ gradient_direction=”left_to_right” shape_divider_position=”bottom” bg_image_animation=”none” gradient_type=”default” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_tablet=”inherit” column_padding_phone=”inherit” column_padding_position=”all” column_element_direction_desktop=”default” column_element_spacing=”default” desktop_text_alignment=”default” tablet_text_alignment=”default” phone_text_alignment=”default” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_backdrop_filter=”none” column_shadow=”none” column_border_radius=”none” column_link_target=”_self” column_position=”default” gradient_direction=”left_to_right” overlay_strength=”0.3″ width=”1\/1″ tablet_width_inherit=”default” animation_type=”default” bg_image_animation=”none” border_type=”simple” column_border_width=”none” column_border_style=”solid”][vc_column_text]\n

Project TECHSCALE (No.\u00a0CZ.02.01.01\/00\/22_008\/0004587) is financed by\u00a0the\u00a0MEYS OP JAC Excellent research programme, supported by\u00a0ERDF\/ESF.<\/em><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

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