{"id":7055,"date":"2025-10-15T12:42:05","date_gmt":"2025-10-15T10:42:05","guid":{"rendered":"https:\/\/www.techscale.cz\/cs\/?p=7055"},"modified":"2025-10-15T12:42:05","modified_gmt":"2025-10-15T10:42:05","slug":"revolucni-senzor-dokaze-menit-ph-uvnitr-zivych-bunek","status":"publish","type":"post","link":"https:\/\/www.techscale.cz\/cs\/2025\/10\/15\/revolucni-senzor-dokaze-menit-ph-uvnitr-zivych-bunek\/","title":{"rendered":"Revolu\u010dn\u00ed senzor dok\u00e1\u017ee m\u011b\u0159it pH uvnit\u0159 \u017eiv\u00fdch bun\u011bk s nev\u00eddanou p\u0159esnost\u00ed"},"content":{"rendered":"[vc_row type=“in_container“ full_screen_row_position=“middle“ column_margin=“default“ column_direction=“default“ column_direction_tablet=“default“ column_direction_phone=“default“ scene_position=“center“ text_color=“dark“ text_align=“left“ row_border_radius=“none“ row_border_radius_applies=“bg“ overflow=“visible“ overlay_strength=“0.3″ gradient_direction=“left_to_right“ shape_divider_position=“bottom“ bg_image_animation=“none“ gradient_type=“default“ shape_type=““][vc_column column_padding=“no-extra-padding“ column_padding_tablet=“inherit“ column_padding_phone=“inherit“ column_padding_position=“all“ column_element_direction_desktop=“default“ column_element_spacing=“default“ desktop_text_alignment=“default“ tablet_text_alignment=“default“ phone_text_alignment=“default“ background_color_opacity=“1″ background_hover_color_opacity=“1″ column_backdrop_filter=“none“ column_shadow=“none“ column_border_radius=“none“ column_link_target=“_self“ column_position=“default“ gradient_direction=“left_to_right“ overlay_strength=“0.3″ width=“1\/1″ tablet_width_inherit=“default“ animation_type=“default“ bg_image_animation=“none“ border_type=“simple“ column_border_width=“none“ column_border_style=“solid“][vc_column_text]\n

Novou generaci nanosenzor\u016f schopn\u00fdch detekovat pH uvnit\u0159 \u017eiv\u00fdch bun\u011bk s dosud nev\u00eddanou citlivost\u00ed a stabilitou vyvinul t\u00fdm v\u011bdc\u016f z CATRIN Univerzity Palack\u00e9ho v Olomouci a V\u0160B \u2013 Technick\u00e9 univerzity Ostrava ve spolupr\u00e1ci s kolegy z Vysok\u00e9ho u\u010den\u00ed technick\u00e9ho v Brn\u011b, Senzory na b\u00e1zi uhl\u00edkov\u00fdch te\u010dek p\u0159edstavili v \u010dasopise Biosensors and Bioelectronics. <\/a><\/strong><\/h2>\n[\/vc_column_text][divider line_type=“No Line“ custom_height=“25″][vc_column_text]Hodnota pH je jedn\u00edm z nej\u010dast\u011bji m\u011b\u0159en\u00fdch chemick\u00fdch parametr\u016f, ale v\u00fdvoj nanometrick\u00fdch senzor\u016f schopn\u00fdch p\u0159esn\u011b zmapovat distribuci a dynamiku pH s vysok\u00fdm prostorov\u00fdm a \u010dasov\u00fdm rozli\u0161en\u00edm z\u016fst\u00e1v\u00e1 v\u00fdznamnou v\u00fdzvou. Takov\u00e9 senzory jsou \u017eivotn\u011b d\u016fle\u017eit\u00e9 pro na\u0161e ch\u00e1p\u00e1n\u00ed \u010detn\u00fdch fyziologick\u00fdch a patologick\u00fdch proces\u016f.<\/p>\n

\u201eHledali jsme zp\u016fsob, jak sledovat d\u011bn\u00ed uvnit\u0159 bun\u011bk s mnohem vy\u0161\u0161\u00ed p\u0159esnost\u00ed, ne\u017e jakou umo\u017e\u0148uj\u00ed sou\u010dasn\u00e9 sondy. Vyvinuli jsme senzor, kter\u00fd je mal\u00fd, jasn\u011b sv\u00edt\u00edc\u00ed, velmi stabiln\u00ed a dok\u00e1\u017ee sledovat zm\u011bny pH v mimo\u0159\u00e1dn\u011b \u0161irok\u00e9m rozmez\u00ed. Senzory nejen z\u00e1\u0159\u00ed pod mikroskopem, ale tak\u00e9 detekuj\u00ed zm\u011bny pH na z\u00e1klad\u011b doby \u017eivotnosti fluorescence, co\u017e je p\u0159esn\u011bj\u0161\u00ed a spolehliv\u011bj\u0161\u00ed parameter ne\u017e b\u011b\u017en\u00e1 intenzita sv\u011btla,\u201d vysv\u011btlil prvn\u00ed autor studie Sergii Kalytchuk z CATRIN a Materi\u00e1lov\u011b-environment\u00e1ln\u00ed laborato\u0159e (MEL) na V\u0160B\u2013TUO.[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=“in_container“ full_screen_row_position=“middle“ column_margin=“default“ column_direction=“default“ column_direction_tablet=“default“ column_direction_phone=“default“ scene_position=“center“ left_padding_desktop=“0″ constrain_group_2=“yes“ right_padding_desktop=“0″ text_color=“dark“ text_align=“left“ row_border_radius=“none“ row_border_radius_applies=“bg“ overflow=“visible“ overlay_strength=“0.3″ gradient_direction=“left_to_right“ shape_divider_position=“bottom“ bg_image_animation=“none“ gradient_type=“default“ shape_type=““][vc_column column_padding=“no-extra-padding“ column_padding_tablet=“inherit“ column_padding_phone=“inherit“ column_padding_position=“all“ column_element_direction_desktop=“default“ column_element_spacing=“default“ desktop_text_alignment=“default“ tablet_text_alignment=“default“ phone_text_alignment=“default“ background_color_opacity=“1″ background_hover_color_opacity=“1″ column_backdrop_filter=“none“ column_shadow=“none“ column_border_radius=“none“ column_link_target=“_self“ column_position=“default“ gradient_direction=“left_to_right“ overlay_strength=“0.3″ width=“1\/2″ tablet_width_inherit=“default“ animation_type=“default“ bg_image_animation=“none“ border_type=“simple“ column_border_width=“none“ column_border_style=“solid“ column_padding_type=“default“ gradient_type=“default“][image_with_animation image_url=“7057″ image_size=“full“ animation_type=“entrance“ animation=“None“ animation_movement_type=“transform_y“ hover_animation=“none“ alignment=“center“ border_radius=“10px“ box_shadow=“none“ image_loading=“default“ max_width=“100%“ max_width_mobile=“default“][\/vc_column][vc_column right_padding_desktop=“5%“ column_element_direction_desktop=“default“ column_element_spacing=“default“ desktop_text_alignment=“default“ tablet_text_alignment=“default“ phone_text_alignment=“default“ background_color_opacity=“1″ background_hover_color_opacity=“1″ column_backdrop_filter=“none“ column_shadow=“none“ column_border_radius=“none“ column_link_target=“_self“ column_position=“default“ gradient_direction=“left_to_right“ overlay_strength=“0.3″ width=“1\/2″ tablet_width_inherit=“default“ animation_type=“default“ bg_image_animation=“none“ border_type=“simple“ column_border_width=“none“ column_border_style=“solid“ column_padding_type=“advanced“ gradient_type=“default“][vc_column_text]Na rozd\u00edl od b\u011b\u017en\u00fdch fluorescen\u010dn\u00edch senzor\u016f pH, kter\u00e9 trp\u00ed fotoblednut\u00edm, chemick\u00fdm rozkladem a \u010dasto vy\u017eaduj\u00ed kalibraci, si nov\u00e9 nanosenzory RhB-CD udr\u017euj\u00ed vynikaj\u00edc\u00ed v\u00fdkon i v n\u00e1ro\u010dn\u00fdch podm\u00ednk\u00e1ch. Doba jejich fluorescence se p\u0159edv\u00eddateln\u011b prodlu\u017euje s rostouc\u00edm pH, co\u017e umo\u017e\u0148uje p\u0159\u00edm\u00e9 m\u011b\u0159en\u00ed bun\u011b\u010dn\u00e9 kyselosti \u2013 kl\u00ed\u010dov\u00e9ho parametru, jeho\u017e v\u00fdkyvy \u00fazce souvis\u00ed s chorobn\u00fdmi procesy, jako je rakovina a neurodegenerace.<\/p>\n

\u201eV experimentu s lidsk\u00fdmi ko\u017en\u00edmi bu\u0148kami a pomoc\u00ed pokro\u010dil\u00e9 mikroskopie zalo\u017een\u00e9 na m\u011b\u0159en\u00ed doby \u017eivotnosti fluorescence (FLIM) umo\u017enily senzory p\u0159esn\u011b zmapovat pH uvnit\u0159 lyzozom\u016f, co\u017e jsou zjednodu\u0161en\u011b \u0159e\u010deno recykla\u010dn\u00ed a \u00faklidov\u00e9 jednotky bu\u0148ky. Nav\u00edc bylo mo\u017en\u00e9 detekovat zm\u011bny pH po aplikaci chemick\u00fdch inhibitor\u016f, co\u017e otev\u00edr\u00e1 mo\u017enosti pro testov\u00e1n\u00ed l\u00e9\u010div i sledov\u00e1n\u00ed stresu v bu\u0148k\u00e1ch v re\u00e1ln\u00e9m \u010dase,\u201d uvedl dal\u0161\u00ed z autor\u016f Tom\u00e1\u0161 Malina.<\/p>\n

My\u0161lenka senzoru vznikla na pomez\u00ed syntetick\u00e9 chemie a fyziky, ale pr\u00e1v\u011b jeho vyu\u017eit\u00ed v biomedic\u00edn\u011b d\u00e1v\u00e1 cel\u00e9 pr\u00e1ci \u0161ir\u0161\u00ed v\u00fdznam. \u201eStudie ukazuje, jak lze propojit z\u00e1kladn\u00ed materi\u00e1lovou chemii se skute\u010dn\u00fdmi biomedic\u00ednsk\u00fdmi v\u00fdzvami. D\u00edky v\u00fdjime\u010dn\u00e9 fotostabilit\u011b, biokompatibilit\u011b a extr\u00e9mn\u011b \u0161irok\u00e9mu pH rozsahu maj\u00ed tyto senzory velk\u00fd potenci\u00e1l st\u00e1t se \u00fa\u010dinn\u00fdm n\u00e1strojem pro z\u00e1kladn\u00ed v\u00fdzkum, diagnostiku i budouc\u00ed klinick\u00e9 aplikace.\u201c uzav\u0159el koresponden\u010dn\u00ed autor \u010dl\u00e1nku Radek Zbo\u0159il.[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=“in_container“ full_screen_row_position=“middle“ column_margin=“default“ column_direction=“default“ column_direction_tablet=“default“ column_direction_phone=“default“ scene_position=“center“ text_color=“dark“ text_align=“left“ row_border_radius=“none“ row_border_radius_applies=“bg“ overflow=“visible“ overlay_strength=“0.3″ gradient_direction=“left_to_right“ shape_divider_position=“bottom“ bg_image_animation=“none“][vc_column column_padding=“no-extra-padding“ column_padding_tablet=“inherit“ column_padding_phone=“inherit“ column_padding_position=“all“ column_element_direction_desktop=“default“ column_element_spacing=“default“ desktop_text_alignment=“default“ tablet_text_alignment=“default“ phone_text_alignment=“default“ background_color_opacity=“1″ background_hover_color_opacity=“1″ column_backdrop_filter=“none“ column_shadow=“none“ column_border_radius=“none“ column_link_target=“_self“ column_position=“default“ gradient_direction=“left_to_right“ overlay_strength=“0.3″ width=“1\/1″ tablet_width_inherit=“default“ animation_type=“default“ bg_image_animation=“none“ border_type=“simple“ column_border_width=“none“ column_border_style=“solid“][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row type=“full_width_content“ full_screen_row_position=“middle“ column_margin=“default“ column_direction=“default“ column_direction_tablet=“default“ column_direction_phone=“default“ scene_position=“center“ top_padding=“2%“ left_padding_desktop=“36%“ constrain_group_2=“yes“ right_padding_desktop=“36%“ text_color=“dark“ text_align=“left“ row_border_radius=“none“ row_border_radius_applies=“bg“ overflow=“visible“ overlay_strength=“0.3″ gradient_direction=“left_to_right“ shape_divider_position=“bottom“ bg_image_animation=“none“ gradient_type=“default“ shape_type=““][vc_column column_padding=“no-extra-padding“ column_padding_tablet=“inherit“ column_padding_phone=“inherit“ column_padding_position=“all“ column_element_direction_desktop=“default“ column_element_spacing=“default“ desktop_text_alignment=“default“ tablet_text_alignment=“default“ phone_text_alignment=“default“ background_color_opacity=“1″ background_hover_color_opacity=“1″ column_backdrop_filter=“none“ column_shadow=“none“ column_border_radius=“none“ column_link_target=“_self“ column_position=“default“ gradient_direction=“left_to_right“ overlay_strength=“0.3″ width=“1\/1″ tablet_width_inherit=“default“ animation_type=“default“ bg_image_animation=“none“ border_type=“simple“ column_border_width=“none“ column_border_style=“solid“][image_with_animation image_url=“6593″ image_size=“full“ animation_type=“entrance“ animation=“None“ animation_movement_type=“transform_y“ hover_animation=“none“ alignment=““ border_radius=“none“ box_shadow=“none“ image_loading=“default“ max_width=“100%“ max_width_mobile=“default“][\/vc_column][\/vc_row][vc_row type=“in_container“ full_screen_row_position=“middle“ column_margin=“default“ column_direction=“default“ column_direction_tablet=“default“ column_direction_phone=“default“ scene_position=“center“ top_padding=“2%“ left_padding_desktop=“10%“ constrain_group_2=“yes“ right_padding_desktop=“10%“ text_color=“dark“ text_align=“left“ row_border_radius=“none“ row_border_radius_applies=“bg“ overflow=“visible“ overlay_strength=“0.3″ gradient_direction=“left_to_right“ shape_divider_position=“bottom“ bg_image_animation=“none“ gradient_type=“default“ shape_type=““][vc_column column_padding=“no-extra-padding“ column_padding_tablet=“inherit“ column_padding_phone=“inherit“ column_padding_position=“all“ column_element_direction_desktop=“default“ column_element_spacing=“default“ desktop_text_alignment=“default“ tablet_text_alignment=“default“ phone_text_alignment=“default“ background_color_opacity=“1″ background_hover_color_opacity=“1″ column_backdrop_filter=“none“ column_shadow=“none“ column_border_radius=“none“ column_link_target=“_self“ column_position=“default“ gradient_direction=“left_to_right“ overlay_strength=“0.3″ width=“1\/1″ tablet_width_inherit=“default“ animation_type=“default“ bg_image_animation=“none“ border_type=“simple“ column_border_width=“none“ column_border_style=“solid“][vc_column_text]\n

Projekt TECHSCALE (No.\u00a0CZ.02.01.01\/00\/22_008\/0004587) je financov\u00e1n z\u00a0programu M\u0160MT\u00a0OP\u00a0JAK \u0160pi\u010dkov\u00fd v\u00fdzkum, podpo\u0159en\u00e9ho z\u00a0EFRR\/ESF.<\/em><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

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